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Resp. Erick Dufourc (This email address is being protected from spambots. You need JavaScript enabled to view it., Tel: +33 5 40 00 68 18)
Corr. Axelle Grélard ( This email address is being protected from spambots. You need JavaScript enabled to view it. , Tel: +33 5 40 00 22 30)

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Institute of Chemistry & Biology of Membranes & Nano-objects / Institut Européen de Chimie et de Biologie

Platform Technical Description:

Spectrometer 800 MHz for applications in Liquid, Solid & Soft matter

Console:
Bruker Avance III, 4 channels

Work Station:
Environnement Linux, software Topspin 3.1

NMR Probes:
Cryoprobe 5mm TCI 1H-13C/15N/2H with Z-gradient, (S/B =x), T= 4-50 °C
CPMAS 4 mm probe 2 channels: 1H/BB
CPMAS 3.2 mm probe 3 channels: 1H/13C/15N (being installed in 2014)

photoNMR experiments available:

Liquid state NMR experiments (all multidimensional experiemnts including those in weakly oriented media).

Soft matter NMR: membranes, colloids or liquid crystals. Wide line, magic angle sample spinning and oriented samples.

Solid state NMR experiments (CPMAS) including fibrilar or microcrystalline proteins

Main domains of interest:

  • NMR of membrane lipids in the context of bicelles, liposomes, membrane domains "rafts", atherosclerosis & cellular signaling (nanoobjets orineted by magnetic fields, sterols, phosphoinositides, etc.)
  • NMR of membrane peptides and proteins involved in cancer, apoptosis or with antimicrobial or antibiotic properties (neu/erbB-2, Bax, Bcl-2, Melittin, Surfactin, Cateslytin, etc.)
  • NMR of natural colloids in health and nutrition domains (tannins/saliva proteins, sprayable lipoaminoacids/active proteins).
  • CP MAS NMR of fibrilar proteins involved in supramolacular complexes.
  • Supramolecular and bioorganic chemistry, foldamers and peptidomimetics
  • Self assembly of amphilphilic molecules
  • Synthesis & Acrivity of natural substances of biological interest  (phenols & quinones)
  • 3D structure of nucleic acids, proteins and their complexes
  • Solid state Chemistry, materials, alloys.
  • 2D-3D multinuclear liquid state NMR
  • RDC (residual dipolar coupling)
  • Relaxation13C15N2H31P

 

Other NMR Equipments on the platform:

700 MHz Bruker Avance III Liquids / Solids

LS NMR Probes:
TXI (1H, 13C, 15N, 2H), 5 mm, z-gradients
BBO (109Ag – 31P, 1H, 2H), 5 mm, z-gradients
HRMAS (1H, 13C, 15N, 2H), 4 mm
SS NMR Probes:
CPMAS (1H, 2H, 31P), 4 mm, E-free
CPMAS (15N – 31P, 1H), 4 mm

500 MHz Bruker Avance II Liquids / Solids

LS NMR Probe:
HRMAS (1H, 13C, 2H), 4 mm, z-gradients

SS NMR Probes:
CPMAS (1H, X, Y), 4 mm
WL Gonio manual (1H, X, Y), rotor de 4, 7 ou 10 mm
CPMAS (1H, X, Y), 2,5 mm, (?r < 33 kHz)
CPMAS (1H, X), 4 mm

400 MHz Bruker Avance DPX Liquide

LS NMR Probes:
QNP (13C-31P-19F, 1H, 2H), 5 mm, z-gradients
BBI (15N-31P, 1H, 2H), 5 mm, z-gradients

300 MHz Bruker Avance I Solide

SS NMR Probes:
CPMAS (1H, X, Y), 4 mm
CPMAS (1H, X, Y), 4 mm, high temperatures (< 300°C)
WL Gonio automatic (1H, X, Y), rotors de 4 ou 7 mm

300 MHz Bruker Avance II Liquide

LS NMR Probe:
BBFO (15N-31P, 1H, 2H), 5 mm, z-gradients

100 MHz Bruker Avance II Solide

SS NMR Probe:
MAS (X, Y), 4 mm

A propos : CBMN & IECB

The institute of Chemistry and Biology of Membranes and Nano-objects (CBMN) is an institute belonging to CNRS, University of Bordeaux and to the Bordeaux Polytechnic Institute. It is multi-disciplinary and operates at the interface between Chemistry, Biology and Physics by hosting 14 research laboratories distributed in three departments: Biomimetic and Therapeutic Chemistry, Biophysical Chemistry and Biology and Biotechnology. 8 Technical Platforms (NMR, X-Rays, Electron Microscopy, Mass spectrometry, Vibrational spectroscopy (IR-Raman-BA-PWR), Molecular Modelling, AFM, and peptide synthesis.) nicely complement the high level expertise of research laboratories. CBMN is part a national plan called “Invest to the Future”: IDEX Bordeaux, Labex AMADEUS, VIBNANO and LISA Carnot Institute. A close partnership is particularly developed with pharmaceutical and formulation industries and also with industries in the nutrition and health domains (lipids and vine/wine products).

The European Institute for Chemistry & Biology (IECB), UMS 3033, is an incubator of international and interdisciplinary research teams at the interface between chemistry and biology. The platform of structural  biophysical chemistry gathers several equipments and expertises available at IECB to answer structural and functionnal questions on molecules of medical interest, with particular emphasis on topics related to biomembranes, supramolecular chemistry and gene transfer.

 

Coming to CBMN/IECB:

 

Some recent publications:

  1. Zhou, J., Murayama, K., Amrane, S., Rosu, F., Kashida, H., Bourdoncle, A., Asanuma, H., and Mergny, J. L.
    (2013) A "sugar-deficient" G-quadruplex: incorporation of aTNA in G4 structures. Chem. Sci. 4, 3693-3698
  2. Zhou, J., Amrane, S., Korkut, D. N., Bourdoncle, A., He, H. Z., Ma, D. L., and Mergny, J. L.
    (2013) Combination of i-Motif and G-Quadruplex Structures within the Same Strand: Formation and Application. Angew. Chem.-Int. Edit. 52, 7742-7746
  3. Uzureau, P., Uzureau, S., Lecordier, L., Fontaine, F., Tebabi, P., Homble, F., Grelard, A., Zhendre, V., Nolan, D. P., Lins, L., Crowet, J.-M., Pays, A., Felu, C., Poelvoorde, P., Vanhollebeke, B., Moestrup, S. K., Lyngso, J., Pedersen, J. S., Mottram, J. C., Dufourc, E. J., Perez-Morga, D., and Pays, E.
    (2013) Mechanism of Trypanosoma brucei gambiense resistance to human serum. Nature 501, 430-+
  4. Urzel, V., Schuliar, Y., Adam, F., Grelard, A., Courreges, C., Dufourc, E. J., and Duday, H.
    (2013) Intra-individual changes in major bone constituents studied by Solid State Nuclear Magnetic Resonance Spectroscopy. Am. J. Phys. Anthropol. 150, 274-275
  5. Tomasini, C., Huc, I., Aitken, D. J., and Fulop, F.
    (2013) Foldamers. Eur. J. Org. Chem. , 3408-3409
  6. Salesse, K., Urzel, V., Dufour, E., Castex, D., Bruzek, J., and Dufourc, E. J.
    (2013) Validation of bone apatite purification protocols for stable isotope analysis in bioarchaeology by Solid-State Nuclear Magnetic Resonance spectroscopy. Am. J. Phys. Anthropol. 150, 239-239
  7. Pendem, N., Nelli, Y. R., Douat, C., Fischer, L., Laguerre, M., Ennifar, E., Kauffmann, B., and Guichard, G.
    (2013) Controlling Helix Formation in the gamma-Peptide Superfamily: Heterogeneous Foldamers with Urea/Amide and Urea/Carbamate Backbones. Angew. Chem.-Int. Edit. 52, 4147-4151
  8. Pendem, N., Douat, C., Claudon, P., Laguerre, M., Castano, S., Desbat, B., Cavagnat, D., Ennifar, E., Kauffmann, B., and Guichard, G.
    (2013) Helix-Forming Propensity of Aliphatic Urea Oligomers Incorporating Noncanonical Residue Substitution Patterns. J. Am. Chem. Soc. 135, 4884-4892
  9. Kudo, M., Maurizot, V., Kauffmann, B., Tanatani, A., and Huc, I.
    (2013) Folding of a Linear Array of alpha-Amino Acids within a Helical Aromatic Oligoamide Frame. J. Am. Chem. Soc. 135, 9628-9631
  10. Jobin, M. L., Bonnafous, P., Temsamani, H., Dole, F., Grelard, A., Dufourc, E. J., and Alves, I. D.
    (2013) The enhanced membrane interaction and perturbation of a cell penetrating peptide in the presence of anionic lipids: Toward an understanding of its selectivity for cancer cells. Biochim. Biophys. Acta-Biomembr. 1828, 1457-1470
  11. Amrane S, Mackereth CD. Protein chemical shift assignments of the unbound and RNA-bound forms of the alternative splicing factor SUP-12 from C. elegans. Biomol NMR Assign. 2013 DOI: 10.1007/s12104-013-9463-9.
  12. Monneau YR, Soufari H, Nelson CJ, Mackereth CD. Structure and Activity of the Peptidyl-Prolyl Isomerase Domain from the Histone Chaperone Fpr4 toward Histone H3 Proline Isomerization. J Biol Chem. 2013 288:25826-37.
  13. Gravel, A. E., Arnold, A. A., Dufourc, E. J., and Marcotte, I.
    (2013) An NMR investigation of the structure, function and role of the hERG channel selectivity filter in the long QT syndrome. Biochim. Biophys. Acta-Biomembr. 1828, 1494-1502
  14. Gudavicius, G., Soufari, H., Upadhyay, S., Mackereth, C. D., and Nelson, C. J.
    (2013) Resolving the functions of peptidylprolyl isomerases: insights from the mutagenesis of the nuclear FKBP25 enzyme. Biochem. Soc. Trans. 41, 761-768
  15. Grélard, A., Guichard, P., Bonnafous, P., Marco, S., Lambert, O., Manin, C., Ronzon, F., and Dufourc, E. J.
    (2013) Hepatitis B subvirus particles display both a fluid bilayer membrane and a strong resistance to freeze drying: a study by solid-state NMR, light scattering, and cryo-electron microscopy/tomography.
    The FASEB Journal, in the press
  16. Berthelot, K., Lecomte, S., Estevez, Y., Zhendre, V., Henry, S., Thévenot, J., Dufourc, E. J., Alves, I. D., and Peruch, F.
    (2013) Rubber particle proteins, HbREF and HbSRPP, show different interactions with model membranes.
    Biochimica et Biophysica Acta (BBA) - Biomembranes In press

 

 

Some 2013 “Highligths”, for which the TGIR RMN THC Bordeaux platform has been used (in french):

Pourquoi les parasites responsables de la maladie du sommeil résistent à la défense immunitaire de l'homme?

Le Yin et le Yang des virus : l’enveloppe de la particule antigénique à la base du vaccin contre l’hépatite B est à la fois fluide et résistante à la lyophilisation